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Image Search Results
Journal: Microbial Cell Factories
Article Title: Three-dimensional tumor spheroids for in vitro analysis of bacteria as gene delivery vectors in tumor therapy
doi: 10.1186/s12934-015-0383-5
Figure Lengend Snippet: Germination of C. sporogenes spores in MCTS. HT-29 MCTS were infected with 1 × 10 3 spores and incubated for 7 days. Cryosections of MCTS were then stained for spores (FITC-conjugated antiserum, green ). Nuclei and DNA of vegetative bacterial cells were stained with Hoechst ( blue ). Images were acquired with a ×10 (in a scale bar 100 µm), or ×100 (in b , c scale bars 10 µm) objective. Higher magnification images in b , c are acquired at locations inside the hypoxic area of MCTS. The inset in the upper right corner of c is a digital zoom on the image part marked with a white box . Images are composite of both fluorescence channels and one representative image of three independent experiments is shown
Article Snippet: Moreover,
Techniques: Infection, Incubation, Staining, Fluorescence